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The Chen Group

Membrane protein biophysics
  Single molecule nanopore sensing
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RESEARCH

The research in the Chen lab is focused on membrane pore-forming proteins. We are interested in both the fundamental principles of protein structure and function relationship and their potential in biotechnological applications.

 

Our current research schemes are as follows:

 

  • New nanopore tools to monitor protein structural dynamics at single molecule level. 

  • Engineering OmpG nanopores for protein sensing.

  • DNA/RNA sequencing.

  • Peptide and protein sequencing. 

  • β-barrel membrane protein folding and design.

RESEARCH
NEWS
NEWS
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PhD Defense 

August 8, 2024

Spencer Shorkey has successfully defended his PhD dissertation entitled "Developing Nanopore Tweezer Technology for Enzymatic Function Analysis". Congratulations, Spencer!

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PhD Defense 

September 27, 2023

Minji Kim has successfully defended her PhD dissertation entitled "Development and evaluation of novel detection techniques for food borne pathogens". Congratulations, Minji! 

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Minji Kim wins
IAFP Technical Talk Competition

May 3, 2023

Congratulations to Minji Kim for winning the International Association for Food Protection European Symposium Technical Talk Competition in Aberdeen, Scotland! 

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PhD Defense 

August 1, 2024

Joshua Foster has successfully defended his PhD dissertation entitled "Expanding the Nanopore Toolbox– Engineering Nanopore Sensors for Bioanalysis". Congratulations, Josh! 

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MS Defense 

July 28, 2023

Congratulations to Samantha Schultz for completion of the Masters Defense!

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New members

January 2023

We have three new members in our laboratory from the Chemistry and MCB PhD Program. Welcome Lily Nguyen, Moly Rani and David DeCoeur!

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CBI Fellow Award

June 2024

Congratulations to David DeCoeur for receiving the NIH Chemistry-Biology Interface Program Fellowship Award! 

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NIH R01 grant to the Aksimentiev, Chen, Niederweiser and Wanunu group

June 1, 2023

Our proposal "Asymmetric Single-Chain MspA nanopores for electroosmotic stretching and sequencing proteins" has been funded by the NIH. Thank you!

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Final Defense
Completion

August 22, 2022

Congratulations to Dr. Fanjun Li for successfully defending her PhD dissertation research entitled “Investigation of kinase conformational dynamics and analytes detection with protein nanopore".

LATEST ARTICLE
LATEST ARTICLES
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ClyA

Abl kinase free energy landscape

Li F, Fahie MA, Gilliam K, Pham R and Chen M: Mapping the energy landscape of Abl kinase by using ClyA nanopore tweezers. Nat Commun, 2022, 13, 3541. 

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ClyA

Maltose binding protein enzymology

Li X, Lee K, Chen J and Chen M*: Different anomeric sugar bound states of MBP resolved by a ClyA nanopore tweezer. ACS Nano 2020, 14(2): 1727–1737.

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Droplet Bilayer

Translocation

across membrane

Li X, Huang J, Holden MA and Chen M: Peptide mediated membrane transport of macromolecular cargo driven by membrane asymmetry. Anal Chem, 2017, 89 (22): 12369–12374.

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OmpG

Nanopore protein sensing

Fahie MAV, Li F, Palmer C, Yoon C and Chen M, Modifying the pH sensitivity of OmpG nanopore for improved detection at acidic pH, Biophysical J, 2022, 121(5): 731-741.

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ClyA

Function of

the beta-tongue

Fahie MA, Liang L, Avelino AR, Pham B, Limpikirati P, Vachet RW and Chen M: Disruption of the open conformation of the transmembrane α-helical barrel in the β-tongue deletion mutants of Cytolysin A. Sci Rep, 2018, 8:3796.

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MspA

Nanopore enzyme kinetics

Shorkey S, Du J, Pham R, Strieter ER and Chen M: Real-time and label-free measurement of deubiquitinase activity with a MspA nanopore. ChemBioChem 2021, 22(7), 2688-2692.

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OmpG

pH-Gating Mechanism

Perez-Rathke A, Fahie MA, Chisholm C, Liang J and Chen M: Mechanism of OmpG pH-dependent gating from loop ensemble and single channel studies. J Am Chem Soc, 2018, 140 (3), 1105–1115.

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Contact
Lab Address
​

Department of Chemistry, 720 LGRT

710 North Pleasant St.

Amherst, MA 01003

Tel: 413-545-2318

Office
​

Department of Chemistry, 865 LGRT

710 North Pleasant St.

Amherst, MA 01003

Tel: 413-545-06830

Contact
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